I am so drained for the past few days.
I felt like if i let go of myself i will just collapse.
2 assignments and one exam for a module spanning 1.5 months
It's too much.

For those who think my assignments are easy,
if it is i wun be dying le.
If you think i'm stupid,
at least i dun act clever to say the assignment is easy but tedious.
Trying reading 10 journals for a week! (if you know what are journals)

For your information, this is wat i am doing for the first assignment

Score E
Sequences producing significant alignments: (Bits) Value

gi72073194refXP_797720.1 PREDICTED: similar to Thymidylat... 483 4e-135
gi57100565refXP_533309.1 PREDICTED: similar to Thymidylat... 293 4e-78
gi73961995refXP_849163.1 PREDICTED: similar to Thymidylat... 293 5e-78

>gi72073194refXP_797720.1 PREDICTED: similar to Thymidylate synthase (TS) (TSase) [Strongylocentrotus
purpuratus]
Length=309

Score = 483 bits (1243), Expect = 4e-135
Identities = 229/241 (95%), Positives = 232/241 (96%), Gaps = 1/241 (0%)
Frame = +1

Query 76 MSVTDTPLQTMDTNLKAAKENGITASTEKMDCEGFMPPPPKHDEDQYLDLIRHIQAHGHV 255
MSVTDTPLQTMDTNLKAAKENGITASTEKMDCEGFMPPPPKHDEDQYLDLIRHIQAHGHV
Sbjct 1 MSVTDTPLQTMDTNLKAAKENGITASTEKMDCEGFMPPPPKHDEDQYLDLIRHIQAHGHV 60

Query 256 KGDRTGTGTKSIFGAQSRYNLRDRFPLLTTKRVFWRGVAEELLWFVKGCTNATELQDKKI 435
KGDRTGTGTKSIFGAQSRYNLRD+FPLLTTKRVFWRGVAEELLWFVKGCTNATELQDKKI
Sbjct 61 KGDRTGTGTKSIFGAQSRYNLRDQFPLLTTKRVFWRGVAEELLWFVKGCTNATELQDKKI 120

Query 436 HIWDANGSKDFLEKRGLGHYEEGDLGPVYGFQWRHFGAEYKDRFTDYTGQGVDQLANVIE 615
HIWDANGSKDFLEKRGLGHYEEGDLGPVYGFQWRHFGAEYKDRFTDYTGQGVDQLANVIE
Sbjct 121 HIWDANGSKDFLEKRGLGHYEEGDLGPVYGFQWRHFGAEYKDRFTDYTGQGVDQLANVIE 180

Query 616 TIKNNPDDRRIIMSAWNPPDLPKRALPPCHAFVQFYVCNGKLSCQLNQNIGKKGVLGSAF 795
TIKNNPDDRRIIMSAWNPPDLPK ALPPCHAFVQFYVCNG+LSCQL Q G G LG F
Sbjct 181 TIKNNPDDRRIIMSAWNPPDLPKMALPPCHAFVQFYVCNGELSCQLYQRSGDMG-LGVPF 239

Query 796 S 798
+
Sbjct 240 N 240 Figure1: Blastx search output (All organism alignment)

Well, dun say you nv learn you dunno. I tot you were very clever? Even ppl like us who attend the modules, the lecturer never teach us how to analyse. He just let us play and do assignment.

For 2nd assignment,

Figure 4: Pyruvate carboxylase with six PfamA domains: CPSase_L_chain (green), CPSase_L_D2 (red), Biotin_carb_C (yellow), HMGL-like (purple), PYC_OADA(turquoise) and Biotin_lipoyl (pale blue)

This is an example of multidomain protein. We are not protein engineers. We are not train to understand how each different amino acids interact to form the ternary structure. All we tried is to understand. You really think it's easy?

Dun ever comment on how easy is other ppl's assignment or work! It is always different when you do yourself. Tell people that it's easy when you have already done something similiar.

THANKS!

Twinkling@
4/19/2006 07:06:00 PM